bacillus selective media ; On Nutrient Agar at 37°C, it forms large (2-5 mm) grey-white, granular colonies with a less wavy edge and less membranous consistency. Engineering Bacillus subtilis for the formation of a durable living ... Bacillus subtilis No or very little growth . . Plate onto selective LB agar plates and incubate overnight at 37 °C. MacConkey Agar (MAC) is a selective and differential medium designed to isolate and differentiate enterics based on their ability to ferment lactose. Transformations of B. subtilis protoplasts were performed using polyethylene glycol and various plasmid DNAs, followed by regeneration of the protoplasts on c‐R5 medium. cereus bacteria tested on various selective media aStrains tested b Mean log CFU/ml ± SD （colony color） e MYP NGKG X-BC TSA Gram-positive bacteria Aerococcus viridans ATCC 10400 ND c ND ND 9.43 ± 0.02 Bacillus circulans ATCC 4516 7.75 ± 0.10 （w） ND ND 7.90 ± 0.06 B. licheniformis ATCC 12759 8.06 ± 0.09 （w） ND ND 8.03 ± 0.03 selective production and characterization of levan by Bacillus subtilis ... Bacillus subtilis when properly cultivated can save garden plants from destruction by disease. General Methods and Strains. Cereus Selective Agar for microbiology MYP Agar ; The optimum temperature for growth varies from 20°C to 40°C, mostly 37°C. . The most widely used among those are MEYP (Mannitol Egg Yolk Polymyxin agar) and PEMBA (Polymyxin Egg yolk Mannitol Bromothymol blue Agar)). o plate on selective media 1 Liter 10 X MN-Medium: 136 g K 2 HPO 4 (x 3 H 2 O) 60 g KH 2 PO 4 10 g Na-citrat (x 2 H 2 O) 10 ml 20ml MNGE-Medium: 920 µl 1,84 ml 10xMN-Medium 8,28 ml 16,56 ml Sterile water 1 ml 2 ml Glucose (20%) 50 µl 100 µl K-Glutamat (40%) Oxoid - Product Detail . Which is the selective media used to isolate Bacillus subtilis from wheat flour? Tbase (NH 4) 2 SO 4 2 g K 2 HPO 4-3H 2 O 18,3 g KH 2 PO 4 6 g NA 3 Citrate-2 H 2 O 1 g QSP 1L H20 MilliQ 2.