Nicotiana benthamiana plants were cultivated at 25/20°C in a greenhouse with a photoperiod of 18 h light/6 h dark. The quantitative characterization of plant-based promoters is essential for optimal control of gene expression in plant chassis. Transient expression of proteins in leaf epidermal cells. Given the emerging importance of transient expression in N. benthamiana for industrial scale protein and product expression, this A, Syringe agroinfiltration was performed to express GFP, hCul1, and hFbxw7 in the leaves of N. benthamiana, followed by foliar spray application of AsA at the indicated concentration at 2-d intervals. A. tumefaciens strains were resuspended in 1 ml induction medium (10 m m MgCl 2, 10 m m MES pH5.6, 150 μ m acetosyringone) and incubated for 2 h at 28°C.
Agrobacterium infiltration of Nicotiana benthamiana for transient ... Nicotiana benthamiana transient Agro-infiltration is an efficient and simple means of achieving transient expression in N. benthamiana. Article CAS Google Scholar Fischer R, Schillberg S, ⦠(a) NbSGT1 silencing efficiency.
Frontiers | Nicotiana benthamiana as a Transient ⦠Because the transiently â¦
Nicotiana benthamiana as a transient expression host to produce ⦠Nicotiana benthamiana, a wild relative of tobacco, has become increasingly popular as a heterologous expression platform for reconstituting plant natural product pathways, because it is amenable to Agrobacterium-mediated transient expression, a scalable and highly flexible process that enables rapid expression of genes and enzymes from other plant species.
Transient Expression in Nicotiana Benthamiana